Am J Physiol Cell Physiol AJP: Cell Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 264: C1231-C1237, 1993;
0363-6143/93 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mohapatra, N. K.
Right arrow Articles by Boat, T. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mohapatra, N. K.
Right arrow Articles by Boat, T. F.

AJP - Cell Physiology, Vol 264, Issue 5 C1231-C1237, Copyright © 1993 by American Physiological Society

ARTICLES

Sulfate concentrations and transport in human bronchial epithelial cells

N. K. Mohapatra, P. W. Cheng, J. C. Parker, A. M. Paradiso, J. R. Yankaskas, R. C. Boucher and T. F. Boat
Department of Pediatrics, University of North Carolina, Chapel Hill 27599-7220.

Inorganic sulfate concentrations in the cytoplasm of human bronchial epithelial cells exceeded levels in the bathing medium under all circumstances tested. Cell sulfate concentrations were directly related to medium sulfate concentrations and inversely related to medium chloride concentrations. In physiological media there was a sulfate compartment of approximately 0.3 mM that exchanged very slowly with extracellular sulfate. In media lacking chloride, sulfate was accumulated by the cells to a level as high as 2 mM. Sulfate uptake was markedly inhibited by external chloride and by stilbene sulfonic acid derivatives but was not affected by sodium in the medium. Efflux of 35SO4(2-) was stimulated by both chloride and sulfate in the bathing medium but inhibited by stilbenes. The following compounds had no effect on sulfate movements: phorbol esters, adenosine 3',5'-cyclic monophosphate derivatives, and okadaic acid. Changes in medium tonicity were likewise without effect. Our results suggest that human bronchial epithelial cells maintain a steady-state disequilibrium for inorganic sulfate. Furthermore, sulfate appears to exist in at least two compartments in the cells: one that is slowly exchangeable with sulfate in the medium and another exchangeable compartment that is of negligible size in physiological media but that becomes very large in media lacking chloride. Sulfate is transported by an anion exchanger of broad specificity that is not influenced by substances known to modulate chloride channels.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
Q. Xie, R. Welch, A. Mercado, M. F. Romero, and D. B. Mount
Molecular characterization of the murine Slc26a6 anion exchanger: functional comparison with Slc26a1
Am J Physiol Renal Physiol, October 1, 2002; 283(4): F826 - F838.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
D. Markovich
Physiological Roles and Regulation of Mammalian Sulfate Transporters
Physiol Rev, October 1, 2001; 81(4): 1499 - 1533.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. L. Sannes, J. Khosla, C.-M. Li, and I. Pagan
Sulfation of extracellular matrices modifies growth factor effects on type II cells on laminin substrata
Am J Physiol Lung Cell Mol Physiol, October 1, 1998; 275(4): L701 - L708.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online