AJP - Cell Physiology, Vol 264, Issue 4 C885-C893, Copyright © 1993 by American Physiological Society

ARTICLES

Lysophosphatidylcholine causes cGMP-dependent verapamil-sensitive Ca2+ influx in vascular smooth muscle cells

L. L. Stoll and A. A. Spector
Department of Anesthesia, University of Iowa, Iowa City 52242.

Lysophosphatidylcholine (lyso-PC) is a vasoactive phospholipid present in oxidized low-density lipoprotein. We used a coculture model of the vascular wall to study its interaction with endothelial cells (EC) and vascular smooth muscle cells (SMC). Lyso-PC was taken up readily by SMC and gradually acylated to phosphatidylcholine. Low concentrations (< or = 1 microM) of lyso-PC present in the interstitial medium of an EC-SMC coculture system were taken up primarily by the SMC. Lyso-PC produced a rapid two- to three-fold increase in SMC guanosine 3',5'-cyclic monophosphate (cGMP) levels, reaching a maximum in 1 min. This increase was associated with decreased SMC proliferation and increased calcium influx. The increase in intracellular calcium was inhibited by verapamil and KT5823, a specific cGMP-dependent kinase inhibitor, while a similar increase was produced by the membrane-permeant cGMP analogue 8-bromoguanosine 3',5'-cyclic monophosphate. These studies suggest that SMC are the primary target for the biological effects of lyso-PC present in the vessel wall and that the responses are mediated by calcium influx, possibly due to opening of a verapamil-sensitive cGMP kinase-dependent channel.

This article has been cited by other articles:

				H. Kume, S. Ito, Y. Ito, and K. Yamaki Role of Lysophosphatidylcholine in the Desensitization of beta -Adrenergic Receptors by Ca2+ Sensitization in Tracheal Smooth Muscle Am. J. Respir. Cell Mol. Biol., September 1, 2001; 25(3): 291 - 298. [Abstract] [Full Text] [PDF]

				X. Fang, N. L. Weintraub, L. L. Stoll, and A. A. Spector Epoxyeicosatrienoic Acids Increase Intracellular Calcium Concentration in Vascular Smooth Muscle Cells Hypertension, December 1, 1999; 34(6): 1242 - 1246. [Abstract] [Full Text] [PDF]

				X. Fang, S. A. Moore, L. L. Stoll, G. Rich, T. L. Kaduce, N. L. Weintraub, and A. A. Spector 14,15-Epoxyeicosatrienoic acid inhibits prostaglandin E2 production in vascular smooth muscle cells Am J Physiol Heart Circ Physiol, December 1, 1998; 275(6): H2113 - H2121. [Abstract] [Full Text] [PDF]

				L. A. Barber, D. F. Spandau, S. C. Rathman, R. C. Murphy, C. A. Johnson, S. W. Kelley, S. A. Hurwitz, and J. B. Travers Expression of the Platelet-activating Factor Receptor Results in Enhanced Ultraviolet B Radiation-induced Apoptosis in a Human Epidermal Cell Line J. Biol. Chem., July 24, 1998; 273(30): 18891 - 18897. [Abstract] [Full Text] [PDF]

				R. L. Davisson, M. D. Travis, J. N. Bates, and S. J. Lewis Hemodynamic Effects of L- and D-S-Nitrosocysteine in the Rat: Stereoselective S-Nitrosothiol Recognition Sites Circ. Res., August 1, 1996; 79(2): 256 - 262. [Abstract] [Full Text]