AJP - Cell Physiology, Vol 264, Issue 4 C783-C788, Copyright © 1993 by American Physiological Society

ARTICLES

Exogenous ATP induces a limited cell cycle progression of arterial smooth muscle cells

R. Malam-Souley, M. Campan, A. P. Gadeau and C. Desgranges
Unite 8 de Cardiologie, Institut National de la Sante et de la Recherche Medicale, Pessac, France.

Because exogenous ATP is suspected to influence the proliferative process, its effects on the cell cycle progression of arterial smooth muscle cells were studied by investigating changes in the mRNA steady-state level of cell cycle-dependent genes. Stimulation of cultured quiescent smooth muscle cells by exogenous ATP induced chronological activation not only of immediate-early but also of delayed-early cell cycle-dependent genes, which were usually expressed after a mitogenic stimulation. In contrast, ATP did not increase late G1 gene mRNA level, demonstrating that this nucleotide induces a limited cell cycle progression of arterial smooth muscle cells through the G1 phase but is not able by itself to induce crossing over the G1-S boundary and consequently DNA synthesis. An increase in c-fos mRNA level was also induced by ADP but not by AMP or adenosine. Moreover, 2-methylthioadenosine 5'-triphosphate but not alpha, beta-methyleneadenosine 5'-triphosphate mediated this kind of response. Taken together, these results demonstrate that extracellular ATP induces the limited progression of arterial smooth muscle cells through the G1 phase via its fixation on P2 gamma receptors.

This article has been cited by other articles:

				S. Zhang, C. V. Remillard, I. Fantozzi, and J. X.-J. Yuan ATP-induced mitogenesis is mediated by cyclic AMP response element-binding protein-enhanced TRPC4 expression and activity in human pulmonary artery smooth muscle cells Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1192 - C1201. [Abstract] [Full Text] [PDF]

				D. Erlinge Extracellular ATP: a central player in the regulation of vascular smooth muscle phenotype. Focus on "Dual role of PKA in phenotype modulation of vascular smooth muscle cells by extracellular ATP" Am J Physiol Cell Physiol, August 1, 2004; 287(2): C260 - C262. [Full Text] [PDF]

				P. J. White, R. Kumari, K. E. Porter, N. J. M. London, L. L. Ng, and M. R. Boarder Antiproliferative effect of UTP on human arterial and venous smooth muscle cells Am J Physiol Heart Circ Physiol, December 1, 2000; 279(6): H2735 - H2742. [Abstract] [Full Text] [PDF]

				M. Hou, S. Moller, L. Edvinsson, and D. Erlinge Cytokines Induce Upregulation of Vascular P2Y2 Receptors and Increased Mitogenic Responses to UTP and ATP Arterioscler. Thromb. Vasc. Biol., September 1, 2000; 20(9): 2064 - 2069. [Abstract] [Full Text] [PDF]

				H. Chaulet, C. Desgranges, M.-A. Renault, F. Dupuch, G. Ezan, F. Peiretti, G. Loirand, P. Pacaud, and A.-P. Gadeau Extracellular Nucleotides Induce Arterial Smooth Muscle Cell Migration Via Osteopontin Circ. Res., October 26, 2001; 89(9): 772 - 778. [Abstract] [Full Text] [PDF]

				X. Pillois, H. Chaulet, I. Belloc, F. Dupuch, C. Desgranges, and A.-P. Gadeau Nucleotide Receptors Involved in UTP-Induced Rat Arterial Smooth Muscle Cell Migration Circ. Res., April 5, 2002; 90(6): 678 - 681. [Abstract] [Full Text] [PDF]