Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 264: C376-C382, 1993;
0363-6143/93 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kaji, D. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kaji, D. M.

AJP - Cell Physiology, Vol 264, Issue 2 C376-C382, Copyright © 1993 by American Physiological Society

ARTICLES

Effect of membrane potential on K-Cl transport in human erythrocytes

D. M. Kaji
Renal Section, Veterans Affairs Medical Center, Bronx 10468.

We examined whether swelling-activated K-Cl cotransport is electrogenic in human erythrocytes. Baseline membrane potential, measured by the change in fluorescence of the carbocyanine dye diS-C3-5, was not different in hypotonically swollen (-7.6 mV) or isosmotically swollen cells (-9.5 mV). We used hemisodium, a new highly selective Na ionophore, in varying concentrations, in the presence of a fixed outwardly directed Na gradient (intracellular Na, 75 mM; external Na, 1 mM) to vary membrane potential over a wide range despite identical K and Cl concentrations. The membrane potential varied between -8 and -90 mV. K influx increased slightly with hyperpolarization in swollen and nonswollen cells. However, the difference between the two fluxes, swelling-activated K influx, a measure of K-Cl cotransport, was unaffected by voltage changes, as was swelling-activated K efflux. We conclude that K-Cl cotransport in human erythrocytes is electroneutral and by inference has a 1:1 stoichiometry.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
M. L. Jennings and M. F. Adame
Direct estimate of 1:1 stoichiometry of K+-Cl{-} cotransport in rabbit erythrocytes
Am J Physiol Cell Physiol, September 1, 2001; 281(3): C825 - C832.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
J. M. Russell
Sodium-Potassium-Chloride Cotransport
Physiol Rev, January 1, 2000; 80(1): 211 - 276.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. Liapis, M. Nag, and D. M. Kaji
K-Cl cotransporter expression in the human kidney
Am J Physiol Cell Physiol, December 1, 1998; 275(6): C1432 - C1437.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Li, V. Jungmann, A. Kiyatkin, and P. S. Low
Prostaglandin E2 Stimulates a Ca2+-dependent K+ Channel in Human Erythrocytes and Alters Cell Volume and Filterability
J. Biol. Chem., August 2, 1996; 271(31): 18651 - 18656.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Sangan, S. R. Brill, S. Sangan, B. Forbush III, and H. J. Binder
Basolateral K-Cl Cotransporter Regulates Colonic Potassium Absorption in Potassium Depletion
J. Biol. Chem., September 29, 2000; 275(40): 30813 - 30816.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online