AJP - Cell Physiology, Vol 263, Issue 3 C623-C627, Copyright © 1992 by American Physiological Society

ARTICLES

Beta 2-adrenoceptor density in fibroblast culture correlates with human NaCl sensitivity

P. Kotanko, O. Hoglinger and F. Skrabal
Department of Internal Medicine, Krankenhaus Barmherzige Bruder, Graz, Austria.

To study salt sensitivity in humans and its relation to expression of adrenoceptors, 20 male normotensive Caucasians were investigated on a diet of 180 mmol NaCl/day followed by 60 mmol NaCl/day over 2 wk and again by 180 mmol NaCl/day over 2 wk, and blood pressure changes were assessed by long-term oscillatory blood pressure monitoring under basal conditions. Individual cell cultures of skin fibroblasts from skin biopsies were also established, and alpha 2- and beta 2-adrenoceptors were measured. Seven subjects were salt sensitive, and the remainder were salt resistant. Cultured skin fibroblasts in salt-sensitive subjects express less than half the number of beta 2-adrenoceptors compared with salt-resistant subjects (65 +/- 12.7 vs. 173 +/- 14.8 fmol/mg, P less than 0.001), and there is a correlation between the absolute rise of blood pressure on a high-salt diet and the density of beta 2-adrenoceptors (r = -0.67, P less than 0.01). It remains to be established whether a reduced in vitro density of beta 2-adrenoceptors in cultured cells is causally related to salt sensitivity in normotensive humans.

This article has been cited by other articles:

				E. M. Snyder, K. C. Beck, N. M. Dietz, J. H. Eisenach, M. J. Joyner, S. T. Turner, and B. D. Johnson Arg16Gly polymorphism of the {beta}2-adrenergic receptor is associated with differences in cardiovascular function at rest and during exercise in humans J. Physiol., February 15, 2006; 571(1): 121 - 130. [Abstract] [Full Text] [PDF]

				K. Bengtsson, M. Orho-Melander, O. Melander, U. Lindblad, J. Ranstam, L. Rastam, and L. Groop {beta}2-Adrenergic Receptor Gene Variation and Hypertension in Subjects With Type 2 Diabetes Hypertension, May 1, 2001; 37(5): 1303 - 1308. [Abstract] [Full Text] [PDF]

				F. C. Luft Molecular Genetics of Salt-Sensitivity and Hypertension Drug Metab. Dispos., April 1, 2001; 29(4): 500 - 504. [Abstract] [Full Text]

				S. Takami, Z. Y. H. Wong, M. Stebbing, and S. B. Harrap Linkage analysis of glucocorticoid and beta 2-adrenergic receptor genes with blood pressure and body mass index Am J Physiol Heart Circ Physiol, April 1, 1999; 276(4): H1379 - H1384. [Abstract] [Full Text] [PDF]

				P. Kotanko, A. Binder, J. Tasker, P. DeFreitas, S. Kamdar, A. J. L. Clark, F. Skrabal, and M. Caulfield Essential Hypertension in African Caribbeans Associates With a Variant of the ß2-Adrenoceptor Hypertension, October 1, 1997; 30(4): 773 - 776. [Abstract] [Full Text]

				L. P. Svetkey, Y.-T. Chen, S. P. McKeown, L. Preis, and A. F. Wilson Preliminary Evidence of Linkage of Salt Sensitivity in Black Americans at the ß2-Adrenergic Receptor Locus Hypertension, April 1, 1997; 29(4): 918 - 922. [Abstract] [Full Text]

				L. P. Svetkey, P. Z. Timmons, O. Emovon, N. B. Anderson, L. Preis, and Y.-T. Chen Association of Hypertension with ß2- and {alpha}2c10-Adrenergic Receptor Genotype Hypertension, June 1, 1996; 27(6): 1210 - 1215. [Abstract] [Full Text]

				S. R. Goldsmith, W. Dachman, Z. Vajo, H. Zafar, P. Kotanko, F. Skrabal, C. C. Lang, C. M. Stein, and A. J.J. Wood Attenuation of Isoproterenol-Mediated Vasodilatation in Blacks N. Engl. J. Med., November 30, 1995; 333(22): 1502 - 1503. [Full Text]