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Am J Physiol Cell Physiol 263: C616-C622, 1992;
0363-6143/92 $5.00
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AJP - Cell Physiology, Vol 263, Issue 3 C616-C622, Copyright © 1992 by American Physiological Society

ARTICLES

Evidence for the involvement of a K+ channel in isosmotic cell shrinking in vestibular dark cells

P. Wangemann, N. Shiga, C. Welch and D. C. Marcus
Cell Physiology Laboratory, Boys Town National Research Hospital, Omaha, Nebraska 68131.

Cell volume changes were measured in dark cells. Isosmotic addition of 21.4 mM K+, Rb+, Cs+, or NH4+ to a control solution containing 3.6 mM K+ caused piretanide-sensitive cell swelling (initial rate for K+, 0.100 +/- 0.005 microns/s; n = 119), suggesting dependence on the Na(+)-Cl(-)-K+ cotransporter. Subsequent isosmotic removal of 21.4 mM K+ caused piretanide-insensitive cell shrinking (initial rate, -0.104 +/- 0.005 microns/s; n = 119), which was inhibited by barium, lidocaine, quinidine, quinine, verapamil, and 4-aminopyridine but not tetraethylammonium (TEA) or glibenclamide, suggesting the involvement of K+ channel(s). Barium, lidocaine, quinine, quinidine, and 4-aminopyridine caused cell swelling in control solution (initial rate for barium, 0.011 +/- 0.004 microns/s; n = 6), suggesting that the K+ channel is also involved in efflux under control conditions. Cell shrinking was slowed by 21.4 mM extracellular K+, Rb+, or Cs+ but unaffected by Na+, Li+, TEA+, or NH4+ (all in the presence of piretanide and compared with N-methyl-D-glucamine), supporting the notion that the efflux mechanism is permeable to and/or inhibited by K+, Rb+, and Cs+. Cell shrinking was slowed by the presumed replacement of intracellular K+ by Cs+ but not by Rb+. Circumstantial evidence suggests that this putative K+ channel is present in the basolateral membrane. The physiological relevance of such a K+ channel might encompass regulatory volume decrease during K+ secretion.


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K. J. Rennie, J. F. Ashmore, and M. J. Correia
Evidence for an Na+-K+-Cl- cotransporter in mammalian type I vestibular hair cells
Am J Physiol Cell Physiol, December 1, 1997; 273(6): C1972 - C1980.
[Abstract] [Full Text] [PDF]


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