Am J Physiol Cell Physiol AJP: Regulatory, Integrative and Comparative Physiology
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Am J Physiol Cell Physiol 262: C724-C730, 1992;
0363-6143/92 $5.00
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AJP - Cell Physiology, Vol 262, Issue 3 C724-C730, Copyright © 1992 by American Physiological Society

ARTICLES

Tetrodotoxin-insensitive sodium channels in a cardiac cell line from a transgenic mouse

A. Sculptoreanu, M. Morton, C. L. Gartside, S. D. Hauschka, W. A. Catterall and T. Scheuer
Department of Pharmacology, University of Washington, Seattle 98195.

The electrophysiological properties of a cardiac cell line (MCM1) originating from a transgenic mouse were characterized. The dominant current in these cells is a sodium current that is insensitive to concentrations of tetrodotoxin (TTX) up to 100 microM. It activates and inactivates rapidly with half-maximal activation at -40 mV and half-maximal inactivation at -79 mV. This sodium current is reduced by agents that increase intracellular adenosine 3',5'-cyclic monophosphate (cAMP) and activate cAMP-dependent protein kinase including isoproterenol, 8-bromo-cAMP, and isobutylmethylxanthine. The phenylalkylamine desmethoxyverapamil blocks the TTX-insensitive sodium current in MCM1 cells in both tonic and use-dependent fashion. Membrane depolarization enhances this block. It is proposed that the TTX-insensitive sodium current in these cells may be similar in origin to the embryonic type of TTX-insensitive sodium current described in other cardiac and skeletal muscle preparations.


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