AJP - Cell Physiology, Vol 262, Issue 3 C563-C568, Copyright © 1992 by American Physiological Society

ARTICLES

Juxtaglomerular cells cultured on a reconstituted basement membrane

B. M. Rayson
Department of Physiology, Cornell University Medical College-New York Hospital, New York 10021.

Circulating renin levels are regulated by release from juxtaglomerular (JG) cells. Here, for the first time, we describe the primary culture of rat juxtaglomerular cells on a reconstituted basement membrane. In addition, primary cultures were transformed with a temperature-sensitive SV40 large T antigen gene to promote the development of a continuous JG cell line. Both primary cultures and transformed JG cells maintain a highly differentiated state and secrete active renin. These preparations now provide a system in which characterization of the cellular mechanisms of regulation of renin synthesis and release is possible.

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