Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 262: C527-C532, 1992;
0363-6143/92 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shetty, M.
Right arrow Articles by Ismail-Beigi, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shetty, M.
Right arrow Articles by Ismail-Beigi, F.

AJP - Cell Physiology, Vol 262, Issue 2 C527-C532, Copyright © 1992 by American Physiological Society

ARTICLES

Enhancement of glucose transport in response to inhibition of oxidative metabolism: pre- and posttranslational mechanisms

M. Shetty, J. N. Loeb and F. Ismail-Beigi
Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032.

Addition of 5 mM sodium azide to Clone 9 cells, a rat liver cell line characterized by intracellular glucose concentrations of less than 10% that of the external medium and limited glycogen stores, results in a 50-80% reduction in cell ATP content within 20 min which then recovers to near-basal levels within 1 h and is subsequently maintained at normal levels for 24 h despite continuing the presence of the inhibitor. Associated with this adaptive response is a striking stimulation of facilitated glucose transport, mediated by the GLUT-1 transporter, that exhibits "early" and "late" phases that appear to be mechanistically different. During the early phase of the response (0-2 h), glucose transport rate is enhanced 12-fold in the absence of any change in cell GLUT-1 or GLUT-1 mRNA content. In contrast, the late phase of the response (8-24 h) is characterized by a further large stimulation of glucose transport (to 1.6 times the 2-h value) that is associated with 2- to 3- and 6- to 10-fold increments in cell GLUT-1 and GLUT-1 mRNA content, respectively. In time course studies an increase in GLUT-1 mRNA content was observed at 4 h and preceded the increment in GLUT-1 which became detectable after 8 h of exposure to azide. A marked induction of GLUT-1 mRNA by azide was also demonstrable in cells incubated in medium containing higher concentrations of glucose (10.6 mM), although the increment was approximately 20% less than when cells were incubated in standard medium (containing 5.6 mM glucose).(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Jing and F. Ismail-Beigi
Critical role of 5'-AMP-activated protein kinase in the stimulation of glucose transport in response to inhibition of oxidative phosphorylation
Am J Physiol Cell Physiol, January 1, 2007; 292(1): C477 - C487.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Jing and F. Ismail-Beigi
Role of 5'-AMP-activated protein kinase in stimulation of glucose transport in response to inhibition of oxidative phosphorylation
Am J Physiol Cell Physiol, February 1, 2006; 290(2): C484 - C491.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. Rubin and F. Ismail-Beigi
Distribution of Glut1 in detergent-resistant membranes (DRMs) and non-DRM domains: effect of treatment with azide
Am J Physiol Cell Physiol, August 1, 2003; 285(2): C377 - C383.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. Lange
Role of microvillar cell surfaces in the regulation of glucose uptake and organization of energy metabolism
Am J Physiol Cell Physiol, January 1, 2002; 282(1): C1 - C26.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D.-Y. Hwang and F. Ismail-Beigi
Stimulation of GLUT-1 glucose transporter expression in response to hyperosmolarity
Am J Physiol Cell Physiol, October 1, 2001; 281(4): C1365 - C1372.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
Z. Lin, J. M. Weinberg, R. Malhotra, S. E. Merritt, L. B. Holzman, and F. C. Brosius III
GLUT-1 reduces hypoxia-induced apoptosis and JNK pathway activation
Am J Physiol Endocrinol Metab, May 1, 2000; 278(5): E958 - E966.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
A. Behrooz and F. Ismail-Beigi
Stimulation of Glucose Transport by Hypoxia: Signals and Mechanisms
Physiology, June 1, 1999; 14(3): 105 - 110.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
C. Depre, J.-L. J. Vanoverschelde, and H. Taegtmeyer
Glucose for the Heart
Circulation, February 2, 1999; 99(4): 578 - 588.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
F. Saker, J. Ybarra, P. Leahy, R. W. Hanson, S. C. Kalhan, and F. Ismail-Beigi
Glycemia-lowering effect of cobalt chloride in the diabetic rat: role of decreased gluconeogenesis
Am J Physiol Endocrinol Metab, June 1, 1998; 274(6): E984 - E991.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Behrooz and F. Ismail-Beigi
Dual Control of glut1 Glucose Transporter Gene Expression by Hypoxia and by Inhibition of Oxidative Phosphorylation
J. Biol. Chem., February 28, 1997; 272(9): 5555 - 5562.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. M. McGowan, S. Police, J. B. Winslow, and P. H. Pekala
Tumor Necrosis Factor-alpha Regulation of Glucose Transporter (GLUT1) mRNA Turnover. CONTRIBUTION OF THE 3prime -UNTRANSLATED REGION OF THE GLUT1 MESSAGE
J. Biol. Chem., January 10, 1997; 272(2): 1331 - 1337.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online