| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Cell Physiology, Vol 262, Issue 1 C171-C181, Copyright © 1992 by American Physiological Society
ARTICLES |
R. J. Dolor, L. M. Hurwitz, Z. Mirza, H. C. Strauss and A. R. Whorton
Department of Pharmacology, Duke University Medical Center, Durham, North Carolina 27710.
We have investigated the role of the intracellular Ca2+ pool in regulating Ca2+ entry into vascular endothelial cells. The intracellular Ca2+ pool was mobilized using either thapsigargin (TG) or 2',5'-di(tert-butyl)-1,4-benzohydroquinone (BHQ), inhibitors of the endoplasmic reticulum Ca(2+)-adenosinetriphosphatase (ATPase). Mobilization of intracellular Ca2+ stores with either inhibitor depleted intracellular Ca2+ and greatly reduced subsequent mobilization of the inositol 1,4,5-trisphosphate (IP3)-sensitive intracellular Ca2+ pool by bradykinin. However, bradykinin-induced mobilization of the IP3-sensitive intracellular Ca2+ pool only partially reduced the subsequent response of cells to TG and BHQ. Mobilization of the intracellular Ca2+ pool by either TG or BHQ led to a concentration-dependent elevation of cytosolic Ca2+ concentrations ([Ca2+]i) without initiating inositol polyphosphate formation. In contrast to the rapidly developing, transient rise in Ca2+ concentration initiated by bradykinin, maximal concentrations of TG and BHQ stimulated a slowly developing, prolonged elevation of [Ca2+]i that required extracellular Ca2+ and could be blocked by extracellular Ni2+. Extracellular Ca2+ entered the cell through an activated cation entry pathway, since bradykinin, TG, and BHQ stimulated Mn2+ and 45Ca2+ entry. Bradykinin-stimulated 45Ca2+ uptake reached a peak within 2 min, whereas 45Ca2+ influx initiated by TG or BHQ continued for at least 8 min. Importantly, the [Ca2+]i response after low concentrations of BHQ was more transient than that seen after TG. The return of [Ca2+]i to basal values after low concentrations of BHQ was associated with reversal of Ca(2+)-ATPase inhibition and refilling of the IP3-sensitive Ca2+ pool. The continued elevation of [Ca2+]i and prolonged Ca2+ entry seen with TG was associated with continued Ca(2+)-ATPase inhibition and an empty IP3-sensitive Ca2+ pool. We conclude that mobilization of intracellular Ca2+ stores induces Ca2+ entry in endothelial cells which continues until the intracellular Ca2+ pool is refilled.
This article has been cited by other articles:
|
|
 |
|
  F.-X. Yi, R. R. Magness, and I. M. Bird Simultaneous imaging of [Ca2+]i and intracellular NO production in freshly isolated uterine artery endothelial cells: effects of ovarian cycle and pregnancy Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2005; 288(1): R140 - R148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-C. Schneider, D. El Kebir, C. Chereau, S. Lanone, X.-L. Huang, A. S. De Buys Roessingh, J.-C. Mercier, J. Dall'Ava-Santucci, and A. T. Dinh-Xuan Involvement of Ca2+/calmodulin-dependent protein kinase II in endothelial NO production and endothelium-dependent relaxation Am J Physiol Heart Circ Physiol, June 1, 2003; 284(6): H2311 - H2319. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Nilius and G. Droogmans Ion Channels and Their Functional Role in Vascular Endothelium Physiol Rev, October 1, 2001; 81(4): 1415 - 1459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Marrelli Mechanisms of endothelial P2Y1- and P2Y2-mediated vasodilatation involve differential [Ca2+]i responses Am J Physiol Heart Circ Physiol, October 1, 2001; 281(4): H1759 - H1766. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Filippi, A. Parenti, S. Donnini, H. J. Granger, A. Fazzini, and F. Ledda {alpha}1D-Adrenoceptors Cause Endothelium-Dependent Vasodilatation in the Rat Mesenteric Vascular Bed J. Pharmacol. Exp. Ther., March 1, 2001; 296(3): 869 - 875. [Abstract] [Full Text]
|
|
|
|
 |
|
 Q. Hu and R. C. Ziegelstein Hypoxia/Reoxygenation Stimulates Intracellular Calcium Oscillations in Human Aortic Endothelial Cells Circulation, November 14, 2000; 102(20): 2541 - 2547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. W. Broeders, P. A. Doevendans, B. C. A. M. Bekkers, R. Bronsaer, E. van Gorsel, J. W. M. Heemskerk, M. G. A. o. Egbrink, E. van Breda, R. S. Reneman, and R. van der Zee Nebivolol: A Third-Generation {beta}-Blocker That Augments Vascular Nitric Oxide Release : Endothelial {beta}2-Adrenergic Receptor-Mediated Nitric Oxide Production Circulation, August 8, 2000; 102(6): 677 - 684. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Lin, K. A. Fagan, K.-X. Li, P. W. Shaul, D. M. F. Cooper, and D. M. Rodman Sustained Endothelial Nitric-oxide Synthase Activation Requires Capacitative Ca2+ Entry J. Biol. Chem., June 9, 2000; 275(24): 17979 - 17985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Pallone, E. P. Silldorff, and Z. Zhang Inhibition of calcium signaling in descending vasa recta endothelia by ANG II Am J Physiol Heart Circ Physiol, April 1, 2000; 278(4): H1248 - H1255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Parenti, S. Filippi, S. Amerini, H. J. Granger, A. Fazzini, and F. Ledda Inositol Phosphate Metabolism and Nitric-Oxide Synthase Activity in Endothelial Cells Are Involved in the Vasorelaxant Activity of Nebivolol J. Pharmacol. Exp. Ther., February 1, 2000; 292(2): 698 - 703. [Abstract] [Full Text]
|
|
|
|
|
|
M. Teubl, K. Groschner, S. D. Kohlwein, B. Mayer, and K. Schmidt Na+/Ca2+ Exchange Facilitates Ca2+-dependent Activation of Endothelial Nitric-oxide Synthase J. Biol. Chem., October 8, 1999; 274(41): 29529 - 29535. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Fransen, C. Katnik, and D. J. Adams ACh- and caffeine-induced Ca2+ mobilization and current activation in rabbit arterial endothelial cells Am J Physiol Heart Circ Physiol, November 1, 1998; 275(5): H1748 - H1758. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Ivey, A. H. Stephenson, and M. I. Townsley Involvement of cytochrome P-450 enzyme activity in the control of microvascular permeability in canine lung Am J Physiol Lung Cell Mol Physiol, October 1, 1998; 275(4): L756 - L763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Ivey, B. J. Roy, and M. I. Townsley Ablation of lung endothelial injury after pacing-induced heart failure is related to alterations in Ca2+ signaling Am J Physiol Heart Circ Physiol, September 1, 1998; 275(3): H844 - H851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Hu, S. Corda, J. L. Zweier, M. C. Capogrossi, and R. C. Ziegelstein Hydrogen Peroxide Induces Intracellular Calcium Oscillations in Human Aortic Endothelial Cells Circulation, January 27, 1998; 97(3): 268 - 275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Yoshimura, T. Oshima, H. Matsuura, T. Inoue, M. Kambe, and G. Kajiyama Differential Effects of Extracellular Mg2+ on Thrombin-Induced and Capacitative Ca2+ Entry in Human Coronary Arterial Endothelial Cells Arterioscler. Thromb. Vasc. Biol., November 1, 1997; 17(11): 3356 - 3361. [Abstract] [Full Text]
|
|
|
|
 |
|
 B. J. Buckley and A. R. Whorton Tunicamycin increases intracellular calcium levels in bovine aortic endothelial cells Am J Physiol Cell Physiol, October 1, 1997; 273(4): C1298 - C1305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. E. Kerper and P. M. Hinkle Cellular Uptake of Lead Is Activated by Depletion of Intracellular Calcium Stores J. Biol. Chem., March 28, 1997; 272(13): 8346 - 8352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Schussler, F. Lantoine, M.-A. Devynck, D. Glotz, and M. David-Dufilho Human Immunoglobulins Inhibit Thrombin-induced Ca2+ Movements and Nitric Oxide Production in Endothelial Cells J. Biol. Chem., October 25, 1996; 271(43): 26473 - 26476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Corda, H. A. Spurgeon, E. G. Lakatta, M. C. Capogrossi, and R. C. Ziegelstein Endoplasmic Reticulum Ca2+ Depletion Unmasks a Caffeine-Induced Ca2+ Influx in Human Aortic Endothelial Cells Circ. Res., November 1, 1995; 77(5): 927 - 935. [Abstract] [Full Text]
|
|
|
|
 |
|
 N. L. Weintraub, A. H. Stephenson, R. S. Sprague, L. McMurdo, and A. J. Lonigro Relationship of Arachidonic Acid Release to Porcine Coronary Artery Relaxation Hypertension, October 1, 1995; 26(4): 684 - 690. [Abstract] [Full Text]
|
|
|
|
|
|
I. Fleming, B. Fisslthaler, and R. Busse Calcium Signaling in Endothelial Cells Involves Activation of Tyrosine Kinases and Leads to Activation of Mitogen-Activated Protein Kinases Circ. Res., April 1, 1995; 76(4): 522 - 529. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
