Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 261: C467-C475, 1991;
0363-6143/91 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Frame, M. D.
Right arrow Articles by Milanick, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Frame, M. D.
Right arrow Articles by Milanick, M. A.

AJP - Cell Physiology, Vol 261, Issue 3 C467-C475, Copyright © 1991 by American Physiological Society

ARTICLES

Mn and Cd transport by the Na-Ca exchanger of ferret red blood cells

M. D. Frame and M. A. Milanick
Department of Physiology, University of Missouri-Columbia, School of Medicine 65212.

Ca influx via the Na-Ca exchanger into ferret red blood cells is easily measured from a Na-free solution; the intracellular Na concentration is normally approximately 150 mM in ferret red blood cells. We have found that Mn and Cd competitively inhibit Ca influx. Mn influx and Cd influx were a saturable function of the divalent cation concentration, consistent with a carrier mechanism. Indeed, the Km (approximately 10 microM) and the Vmax (usually 1-3 mmol.l packed cells-1.h-1) were similar for Ca, Cd, and Mn. Extracellular Na inhibited divalent cation influx, and intracellular Na stimulated influx. These results are consistent with Na-Cd and Na-Mn influx pathways in ferret red blood cells. Ca (1 mM) almost completely inhibited Mn influx and Cd influx, whereas 1 mM Mg inhibited 5-15%. These results strongly support the notion that Mn and Cd are alternative substrates for Ca on the ferret red cell Na-Ca exchanger. The similarity in the behavior of all three divalent cation places important constraints on kinetic and structural models of the exchanger.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
L. C. Ng, B. D. Kyle, A. R. Lennox, X.-M. Shen, W. J. Hatton, and J. R. Hume
Cell culture alters Ca2+ entry pathways activated by store-depletion or hypoxia in canine pulmonary arterial smooth muscle cells
Am J Physiol Cell Physiol, January 1, 2008; 294(1): C313 - C323.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
T. Yanagiya, N. Imura, S. Enomoto, Y. Kondo, and S. Himeno
Suppression of a High-Affinity Transport System for Manganese in Cadmium-Resistant Metallothionein-Null Cells
J. Pharmacol. Exp. Ther., March 1, 2000; 292(3): 1080 - 1086.
[Abstract] [Full Text]

Home page
 Physiol. Rev.Home page
M. P. Blaustein and W. J. Lederer
Sodium/Calcium Exchange: Its Physiological Implications
Physiol Rev, July 1, 1999; 79(3): 763 - 854.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. L. Sensi, L. M. T. Canzoniero, S. P. Yu, H. S. Ying, J.-Y. Koh, G. A. Kerchner, and D. W. Choi
Measurement of Intracellular Free Zinc in Living Cortical Neurons: Routes of Entry
J. Neurosci., December 15, 1997; 17(24): 9554 - 9564.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online