AJP - Cell Physiology, Vol 261, Issue 1 C77-C85, Copyright © 1991 by American Physiological Society

ARTICLES

Effect of angiotensin II on cytosolic free calcium in neonatal rat cardiomyocytes

D. C. Kem, E. I. Johnson, A. M. Capponi, D. Chardonnens, U. Lang, B. Blondel, H. Koshida and M. B. Vallotton
Department of Medicine, University of Geneva, Switzerland.

The effect of angiotensin II (ANG II) on cytosolic free Ca2+ concentration ([Ca2+]i) was studied in cultured neonatal rat ventricular myocytes. [Ca2+]i was estimated in groups of one to three cells by dual-wavelength microfluorometry or in cell populations using conventional fluorometry. ANG II (10(-8) M) produced an acute short-lived increase over the control basal diastolic [Ca2+]i and increased the frequency of the [Ca2+]i transients. The amplitude of the [Ca2+]i transients was decreased to 64.4% of basal values. The effect of ANG II on [Ca2+]i was blocked by the selective AT1 receptor subtype antagonist Du Pont 753 but not by the AT2 antagonist PD 123319. Removal of extracellular Ca2+ or blockade of voltage-gated Ca2+ channels in cells cultured for 5-7 days abolished the [Ca2+]i transients, but only partially diminished the effect of ANG II on [Ca2+]i. Thapsigargin, an inhibitor of sarcoplasmic reticulum Ca(2+)-Mg(2+)-ATPase, reduced or abolished the [Ca2+]i response to ANG II. Phorbol 12-myristate 13-acetate (PMA), 10(-6) and 10(-7) M, also decreased the amplitude of the Ca2+ transients similar to ANG II. Pretreatment with 10(-6) M PMA or 10(-6) M 1-oleoyl-2-acetyl-glycerol (OAG) inhibited the initial rise in [Ca2+]i and the Ca2+ transients. Thus ANG II produces an acute rise in [Ca2+]i which is derived predominantly from sarcoplasmic reticulum intracellular stores. This acute effect is followed by a significant reduction in the amplitude for the Ca2+ transient and may be mediated by activation of protein kinase C.

This article has been cited by other articles:

				T. Nagy, V. Champattanachai, R. B. Marchase, and J. C. Chatham Glucosamine inhibits angiotensin II-induced cytoplasmic Ca2+ elevation in neonatal cardiomyocytes via protein-associated O-linked N-acetylglucosamine Am J Physiol Cell Physiol, January 1, 2006; 290(1): C57 - C65. [Abstract] [Full Text] [PDF]

				P. Liu, D. A. Misurski, and V. Gopalakrishnan Cysteinyl leukotriene-dependent [Ca2+]i responses to angiotensin II in cardiomyocytes Am J Physiol Heart Circ Physiol, April 1, 2003; 284(4): H1269 - H1276. [Abstract] [Full Text] [PDF]

				E. C. Rothstein, K. L. Byron, R. E. Reed, L. Fliegel, and P. A. Lucchesi H2O2-induced Ca2+ overload in NRVM involves ERK1/2 MAP kinases: role for an NHE-1-dependent pathway Am J Physiol Heart Circ Physiol, August 1, 2002; 283(2): H598 - H605. [Abstract] [Full Text] [PDF]

				D. J. Church, M. C. Rebsamen, D. Morabito, V. van der Bent, M. B. Vallotton, and U. Lang Role of cell contractions in cAMP-induced cardiomyocyte atrial natriuretic peptide release Am J Physiol Heart Circ Physiol, January 1, 2000; 278(1): H117 - H125. [Abstract] [Full Text] [PDF]

				R. M. Touyz, J. Fareh, G. Thibault, and E. L. Schiffrin Intracellular Ca2+ Modulation by Angiotensin II and Endothelin-1 in Cardiomyocytes and Fibroblasts From Hypertrophied Hearts of Spontaneously Hypertensive Rats Hypertension, November 1, 1996; 28(5): 797 - 805. [Abstract] [Full Text]

				R. M. Touyz, P. Sventek, R. Lariviere, G. Thibault, J. Fareh, T. Reudelhuber, and E. L. Schiffrin Cytosolic Calcium Changes Induced by Angiotensin II in Neonatal Rat Atrial and Ventricular Cardiomyocytes Are Mediated via Angiotensin II Subtype 1 Receptors Hypertension, May 1, 1996; 27(5): 1090 - 1096. [Abstract] [Full Text]

				J. Fareh, R. M. Touyz, E. L. Schiffrin, and G. Thibault Endothelin-1 and Angiotensin II Receptors in Cells From Rat Hypertrophied Heart : Receptor Regulation and Intracellular Ca2+ Modulation Circ. Res., February 1, 1996; 78(2): 302 - 311. [Abstract] [Full Text]

				M. Zhang, D. Turnbaugh, D. Cofie, S. Dogan, H. Koshida, R. Fugate, and D. C. Kem Protein Kinase C Modulation of Cardiomyocyte Angiotensin II and Vasopressin Receptor Desensitization Hypertension, February 1, 1996; 27(2): 269 - 275. [Abstract] [Full Text]

				J. Fareh, R. M. Touyz, G. Thibault, and E. L. Schiffrin Basal and Angiotensin II–Induced Cytosolic Free Calcium in Adult Rat Cardiomyocytes and Fibroblasts After Volume Overload Hypertension, December 1, 1995; 26(6): 1149 - 1153. [Abstract] [Full Text]

				J. Sadoshima, Z. Qiu, J. P. Morgan, and S. Izumo Angiotensin II and Other Hypertrophic Stimuli Mediated by G Protein–Coupled Receptors Activate Tyrosine Kinase, Mitogen-Activated Protein Kinase, and 90-kD S6 Kinase in Cardiac Myocytes : The Critical Role of Ca2+-Dependent Signaling Circ. Res., January 1, 1995; 76(1): 1 - 15. [Abstract] [Full Text]

				H.-J. Wang, Y.-C. Zhu, and T. Yao Effects of all-trans retinoic acid on angiotensin II-induced myocyte hypertrophy J Appl Physiol, May 1, 2002; 92(5): 2162 - 2168. [Abstract] [Full Text] [PDF]