Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 261: C17-C22, 1991;
0363-6143/91 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gu, H.
Right arrow Articles by LaBelle, E. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gu, H.
Right arrow Articles by LaBelle, E. F.

AJP - Cell Physiology, Vol 261, Issue 1 C17-C22, Copyright © 1991 by American Physiological Society

ARTICLES

Rapid increase in inositol phosphate levels in norepinephrine-stimulated vascular smooth muscle

H. Gu, H. Martin, R. J. Barsotti and E. F. LaBelle
Bockus Research Institute, Graduate Hospital, Philadelphia, Pennsylvania 19146.

We examined the correlation between agonist-stimulated increases in inositol phosphates and force development in vascular smooth muscle. Segments of rat tail artery were preincubated with [3H]inositol and treated with norepinephrine (10(-5) M) for 3-10 s. Tissue levels of inositol monophosphate (IP), inositol bisphosphate (IP2), and inositol trisphosphate (IP3) were measured. IP and IP2 increased significantly after 3 s of norepinephrine treatment. IP3 increased significantly after 5 s of norepinephrine treatment. Analysis of tissue extracts by high-pressure liquid chromatography demonstrated that the only isomer of IP3 present in any tissue extract was the 1,4,5-isomer [Ins(1,4,5)P3]. Contractile response to norepinephrine stimulation showed that the increase in inositol phosphates coincides well with the time course of force development. This is the first report demonstrating such an early increase in Ins(1,4,5)P3 in agonist-stimulated vascular smooth muscle. These results are consistent with the hypothetical role of Ins(1,4,5)P3 as a mediator linking agonist-receptor activation to increased intracellular calcium and force development in norepinephrine-stimulated vascular smooth muscle.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
M. Salomonsson and W. J. Arendshorst
Calcium recruitment in renal vasculature: NE effects on blood flow and cytosolic calcium concentration
Am J Physiol Renal Physiol, May 1, 1999; 276(5): F700 - F710.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
E. F. LaBelle and E. Polyak
Norepinephrine stimulates arachidonic acid release from vascular smooth muscle via activation of cPLA2
Am J Physiol Cell Physiol, April 1, 1998; 274(4): C1129 - C1137.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online