Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 260: C715-C720, 1991;
0363-6143/91 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Powell, S. K.
Right arrow Articles by Rodriguez-Boulan, E. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Powell, S. K.
Right arrow Articles by Rodriguez-Boulan, E. J.

AJP - Cell Physiology, Vol 260, Issue 4 C715-C720, Copyright © 1991 by American Physiological Society

ARTICLES

Thy-1 expresses two signals for apical localization in epithelial cells

S. K. Powell, M. P. Lisanti and E. J. Rodriguez-Boulan
Department of Cell Biology and Anatomy, Cornell University Medical College, New York, New York 10021.

Recent work has shown that anchoring via glycosyl phosphatidylinositol (GPI) results in apical targeting for a variety of endogenous and transfected plasma membrane proteins expressed in epithelial cells. To further determine the correlation between GPI anchoring and apical localization, we expressed GPI-anchored and secretory forms of Thy-1 in Madin-Darby canine kidney cells by transfection. Native GPI-anchored Thy-1, normally expressed in thymocytes and neurons, was localized to the apical surface. A truncated form of Thy-1, lacking 22 out of 31 hydrophobic amino acids at the COOH-terminus, was also constructed; this deletion blocked attachment of the GPI anchor and resulted in apical secretion of Thy-1. In combination with previous results, our observations indicate that Thy-1 contains apical targeting information in its protein sequence as well as in the GPI anchor.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
B. A. Potter, R. P. Hughey, and O. A. Weisz
Role of N- and O-glycans in polarized biosynthetic sorting
Am J Physiol Cell Physiol, January 1, 2006; 290(1): C1 - C10.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
C. Lipardi, L. Nitsch, and C. Zurzolo
Detergent-insoluble GPI-anchored Proteins Are Apically Sorted in Fischer Rat Thyroid Cells, but Interference with Cholesterol or Sphingolipids Differentially Affects Detergent Insolubility and Apical Sorting
Mol. Biol. Cell, February 1, 2000; 11(2): 531 - 542.
[Abstract] [Full Text]

Home page
 JCBHome page
J. H. Benting, A. G. Rietveld, and K. Simons
N-Glycans Mediate the Apical Sorting of a GPI-anchored, Raft-associated Protein in Madin-Darby Canine Kidney Cells
J. Cell Biol., July 26, 1999; 146(2): 313 - 320.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
C. YEAMAN, K. K. GRINDSTAFF, and W. J. NELSON
New Perspectives on Mechanisms Involved in Generating Epithelial Cell Polarity
Physiol Rev, January 1, 1999; 79(1): 73 - 98.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
C. Yeaman, A. H. L. Gall, A. N. Baldwin, L. Monlauzeur, A. L. Bivic, and E. Rodriguez-Boulan
The O-glycosylated Stalk Domain Is Required for Apical Sorting of Neurotrophin Receptors in Polarized MDCK Cells
J. Cell Biol., November 17, 1997; 139(4): 929 - 940.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online