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AJP - Cell Physiology, Vol 260, Issue 3 C570-C580, Copyright © 1991 by American Physiological Society
ARTICLES |
R. C. Piper, L. J. Hess and D. E. James
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110.
The insulin-regulatable glucose transporter (IRGT) is specifically expressed in muscle and fat cells and undergoes translocation from an intracellular compartment to the cell surface following acute insulin treatment. This study examined sorting differences between the IRGT and the homologous HepG2/erythrocyte/brain glucose transporter (HepG2 GT) when expressed together in insulin-responsive 3T3-L1 adipocytes. The ratio of the amount of transporter per unit protein in the plasma membrane fraction vs. the intracellular membrane fraction was 1:2 for the HepG2 GT and 1:30 for the IRGT. Insulin treatment increased the plasma membrane concentration of the IRGT by 10-fold and the HepG2 GT by 3.5-fold. This distribution was confirmed by confocal immunofluorescence microscopy. Differential sorting within intracellular organelles was evident by sucrose gradient analysis and immunoisolation of transporter vesicles and by double immunofluorescence labeling. We propose that differential sorting at an intracellular locus preferably withdraws the IRGT from a pathway which is in close communication with the plasma membrane, thus allowing the IRGT to regulate glucose entry into fat and muscle cells in a highly insulin-regulated fashion.
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J. T. Tellam, S. L. Macaulay, S. McIntosh, D. R. Hewish, C. W. Ward, and D. E. James Characterization of Munc-18c and Syntaxin-4 in 3T3-L1 Adipocytes. PUTATIVE ROLE IN INSULIN-DEPENDENT MOVEMENT OF GLUT-4 J. Biol. Chem., March 7, 1997; 272(10): 6179 - 6186. [Abstract] [Full Text] [PDF]
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A. Janez, D. S. Worrall, T. Imamura, P. M. Sharma, and J. M. Olefsky The Osmotic Shock-induced Glucose Transport Pathway in 3T3-L1 Adipocytes Is Mediated by Gab-1 and Requires Gab-1-associated Phosphatidylinositol 3-Kinase Activity for Full Activation J. Biol. Chem., August 25, 2000; 275(35): 26870 - 26876. [Abstract] [Full Text] [PDF]
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R. C. Hresko and M. Mueckler A Novel 68-kDa Adipocyte Protein Phosphorylated on Tyrosine in Response to Insulin and Osmotic Shock J. Biol. Chem., June 9, 2000; 275(24): 18114 - 18120. [Abstract] [Full Text] [PDF]
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A. Guilherme, M. Emoto, J. M. Buxton, S. Bose, R. Sabini, W. E. Theurkauf, J. Leszyk, and M. P. Czech Perinuclear Localization and Insulin Responsiveness of GLUT4 Requires Cytoskeletal Integrity in 3T3-L1 Adipocytes J. Biol. Chem., December 1, 2000; 275(49): 38151 - 38159. [Abstract] [Full Text] [PDF]
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A. L. Olson, A. R. Trumbly, and G. V. Gibson Insulin-mediated GLUT4 Translocation Is Dependent on the Microtubule Network J. Biol. Chem., March 30, 2001; 276(14): 10706 - 10714. [Abstract] [Full Text] [PDF]
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J. P. Whitehead, J. C. Molero, S. Clark, S. Martin, G. Meneilly, and D. E. James The Role of Ca2+ in Insulin-stimulated Glucose Transport in 3T3-L1 Cells J. Biol. Chem., July 20, 2001; 276(30): 27816 - 27824. [Abstract] [Full Text] [PDF]
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M. Fujishiro, Y. Gotoh, H. Katagiri, H. Sakoda, T. Ogihara, M. Anai, Y. Onishi, H. Ono, M. Funaki, K. Inukai, et al. MKK6/3 and p38 MAPK Pathway Activation Is Not Necessary for Insulin-induced Glucose Uptake but Regulates Glucose Transporter Expression J. Biol. Chem., June 1, 2001; 276(23): 19800 - 19806. [Abstract] [Full Text] [PDF]
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