AJP - Cell Physiology, Vol 260, Issue 2 C226-C233, Copyright © 1991 by American Physiological Society

ARTICLES

Immunolocalization of gluco- and mineralocorticoid receptors in rabbit kidney

N. Farman, M. E. Oblin, M. Lombes, F. Delahaye, H. M. Westphal, J. P. Bonvalet and J. M. Gasc
Institut National de la Sante et de la Recherche Medicale (INSERM) U. 246, Departement de Biologie, CEN Saclay, Gif-sur-Yvette, France.

The localization of glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) was determined in the rabbit kidney by immunohistochemistry with the use of a monoclonal, anti-GR antibody and a monoclonal, anti-idiotypic, anti-MR antibody. Immunostaining was performed on serial histological sections from normal and adrenalectomized rabbits. The specificity of immunostaining was assessed for MR by in situ competition studies with steroids and for GR by presaturation of the antibody with GR preparation. Immunostaining by both the anti-MR and the anti-GR antibodies was present in all parts of the distal nephron (beyond proximal tubule) and absent in the glomerulus and proximal tubule. The absence of staining by the anti-GR antibody in the proximal tubule suggests that the effects of glucocorticoids in this structure involve either a GR different from that of distal structures or a non-receptor mediated mechanism of action. MR immunostaining predominates in the distal and all along the collecting tubule in its cortical, medullary, and papillary portions. GR immunostaining was most abundant in the medullary ascending limb and distal tubule. Immunostaining by both antibodies was present in papillary interstitial cells and cells of the epithelium lining the papilla. Fifteen to twenty percent of the cells of the cortical collecting tubule, presumably intercalated cells, were devoid of MR and GR immunostaining. Immunostaining was present in both nuclear and cytoplasmic cell compartments. No clear difference was observed between normal and adrenalectomized rabbits. This study is the first report on renal immunolocalization of GR compared with MR. In addition, we show evidence for new targets for corticosteroid hormones such as papillary interstitial cells and papillary epithelium.

This article has been cited by other articles:

				G.-S. Lee, K.-C. Choi, and E.-B. Jeung Glucocorticoids differentially regulate expression of duodenal and renal calbindin-D9k through glucocorticoid receptor-mediated pathway in mouse model Am J Physiol Endocrinol Metab, February 1, 2006; 290(2): E299 - E307. [Abstract] [Full Text] [PDF]

				B. Yao, R. C. Harris, and M.-Z. Zhang Interactions between 11{beta}-hydroxysteroid dehydrogenase and COX-2 in kidney Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2005; 288(6): R1767 - R1773. [Abstract] [Full Text] [PDF]

				C. A. Wagner, K. E. Finberg, S. Breton, V. Marshansky, D. Brown, and J. P. Geibel Renal Vacuolar H+-ATPase Physiol Rev, October 1, 2004; 84(4): 1263 - 1314. [Abstract] [Full Text] [PDF]

				R. Norregaard, T. R. Uhrenholt, C. Bistrup, O. Skott, and B. L. Jensen Stimulation of 11-{beta}-hydroxysteroid dehydrogenase type 2 in rat colon but not in kidney by low dietary NaCl intake Am J Physiol Renal Physiol, August 1, 2003; 285(2): F348 - F358. [Abstract] [Full Text] [PDF]

				D. W. Good, T. George, and B. A. Watts III Aldosterone inhibits HCO-3 absorption via a nongenomic pathway in medullary thick ascending limb Am J Physiol Renal Physiol, October 1, 2002; 283(4): F699 - F706. [Abstract] [Full Text] [PDF]

				C. P. VIO, S.-J. AN, C. CESPEDES, J. C. MCGIFF, and N. R. FERRERI Induction of Cyclooxygenase-2 in Thick Ascending Limb Cells by Adrenalectomy J. Am. Soc. Nephrol., April 1, 2001; 12(4): 649 - 658. [Abstract] [Full Text] [PDF]

				N. Farman and M.-E. Rafestin-Oblin Multiple aspects of mineralocorticoid selectivity Am J Physiol Renal Physiol, February 1, 2001; 280(2): F181 - F192. [Abstract] [Full Text] [PDF]

				S. Muto Potassium Transport in the Mammalian Collecting Duct Physiol Rev, January 1, 2001; 81(1): 85 - 116. [Abstract] [Full Text] [PDF]

				E. Feraille and A. Doucet Sodium-Potassium-Adenosinetriphosphatase-Dependent Sodium Transport in the Kidney: Hormonal Control Physiol Rev, January 1, 2001; 81(1): 345 - 418. [Abstract] [Full Text] [PDF]

				F.-X. Beck, W. Neuhofer, and E. Muller Molecular chaperones in the kidney: distribution, putative roles, and regulation Am J Physiol Renal Physiol, August 1, 2000; 279(2): F203 - F215. [Abstract] [Full Text] [PDF]

				S. Berger, M. Bleich, W. Schmid, T. J. Cole, J. Peters, H. Watanabe, W. Kriz, R. Warth, R. Greger, and G. Schutz Mineralocorticoid receptor knockout mice: Pathophysiology of Na+ metabolism PNAS, August 4, 1998; 95(16): 9424 - 9429. [Abstract] [Full Text] [PDF]

				G. Fejes-Toth, D. Pearce, and A. Naray-Fejes-Toth Subcellular localization of mineralocorticoid receptors in living cells: Effects of receptor agonists and antagonists PNAS, March 17, 1998; 95(6): 2973 - 2978. [Abstract] [Full Text] [PDF]

				E. Feifel, M. Krall, J. P. Geibel, and W. Pfaller Differential activities of H+ extrusion systems in MDCK cells due to extracellular osmolality and pH Am J Physiol Renal Physiol, October 1, 1997; 273(4): F499 - F506. [Abstract] [Full Text] [PDF]

				M. Lombes, N. Alfaidy, E. Eugene, A. Lessana, N. Farman, and J.-P. Bonvalet Prerequisite for Cardiac Aldosterone Action : Mineralocorticoid Receptor and 11ß-Hydroxysteroid Dehydrogenase in the Human Heart Circulation, July 15, 1995; 92(2): 175 - 182. [Abstract] [Full Text]

				A. Attmane-Elakeb, V. Sibella, C. Vernimmen, X. Belenfant, S. C. Hebert, and M. Bichara Regulation by Glucocorticoids of Expression and Activity of rBSC1, the Na+-K+(NH4+)-2Cl- Cotransporter of Medullary Thick Ascending Limb J. Biol. Chem., October 20, 2000; 275(43): 33548 - 33553. [Abstract] [Full Text] [PDF]

				A. Odermatt, P. Arnold, and F. J. Frey The Intracellular Localization of the Mineralocorticoid Receptor Is Regulated by 11beta -Hydroxysteroid Dehydrogenase Type 2 J. Biol. Chem., July 20, 2001; 276(30): 28484 - 28492. [Abstract] [Full Text] [PDF]