Am J Physiol Cell Physiol AJP: Heart and Circulatory Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 259: C675-C686, 1990;
0363-6143/90 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Neylon, C. B.
Right arrow Articles by Irvine, R. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Neylon, C. B.
Right arrow Articles by Irvine, R. F.

AJP - Cell Physiology, Vol 259, Issue 4 C675-C686, Copyright © 1990 by American Physiological Society

ARTICLES

Spatial dynamics of intracellular calcium in agonist-stimulated vascular smooth muscle cells

C. B. Neylon, J. Hoyland, W. T. Mason and R. F. Irvine
Department of Biochemistry, AFRC Institute of Animal Physiology and Genetics Research, Cambridge, United Kingdom.

Vasoconstrictor agonists stimulate smooth muscle contraction by inducing a rise in intracellular free Ca2+. Digital-imaging microscopy of fura-2 fluorescence from single vascular smooth muscle cells cultured from the human internal mammary artery has allowed us to record the subcellular alterations in Ca2+ that occur immediately after stimulation by receptor agonists. The thrombin-induced rise in cytoplasmic free Ca2+ begins in a discrete region typically located close to the end of the cell. Subsequently, this region of elevated Ca2+ expands until Ca2+ is elevated throughout the cell cytoplasm. The rate of spreading in the region of elevated Ca2+ in a linear direction averaged 10.1 microns/s, enabling it to traverse the length of most cells within approximately 5 s, and involved rises in Ca2+ of between 200 and 500 nM. In some cells, the Ca2+ rise began at both ends and collided midway. Similar dynamic changes in the spatial distribution of Ca2+ were recorded in cells stimulated by acetylcholine. The novel observation that vasoconstrictor agonists induce an elevation of Ca2+ in a localized region which subsequently expands throughout the cytoplasm of single smooth muscle cells may provide new insight into the nature of Ca2+ signaling in vascular tissue.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. R. Kovac, T. Chrones, and S. M. Sims
Temporal and spatial dynamics underlying capacitative calcium entry in human colonic smooth muscle
Am J Physiol Gastrointest Liver Physiol, January 1, 2008; 294(1): G88 - G98.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
H. Ishikawa, M. Sasaki, S. Noda, and Y. Koga
Apoptosis Induction by the Binding of the Carboxyl Terminus of Human Immunodeficiency Virus Type 1 gp160 to Calmodulin
J. Virol., August 1, 1998; 72(8): 6574 - 6580.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. G. J. Hoenderop, A. Hartog, P. H. G. M. Willems, and R. J. M. Bindels
Adenosine-stimulated Ca2+ reabsorption is mediated by apical A1 receptors in rabbit cortical collecting system
Am J Physiol Renal Physiol, April 1, 1998; 274(4): F736 - F743.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
H. Karaki, H. Ozaki, M. Hori, M. Mitsui-Saito, K.-I. Amano, K.-I. Harada, S. Miyamoto, H. Nakazawa, K.-J. Won, and K. Sato
Calcium Movements, Distribution, and Functions in Smooth Muscle
Pharmacol. Rev., June 1, 1997; 49(2): 157 - 230.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online