AJP - Cell Physiology, Vol 257, Issue 4 C810-C816, Copyright © 1989 by American Physiological Society

ARTICLES

Administration of a creatine analogue induces isomyosin transitions in muscle

T. S. Moerland, N. G. Wolf and M. J. Kushmerick
Department of Radiology, Harvard Medical School, Boston, Massachusetts.

A creatine analogue, beta-guanidinopropionic acid (beta-GPA), was administered in the food (2% wt/wt) and the water (0.5% wt/vol) of male CD-1 mice. Uptake of the phosphorylated analogue and depletion of phosphocreatine in hindlimb muscle was monitored by 31P nuclear magnetic resonance and was found to be complete within 7 wk. After this time, the isomyosin composition of soleus, extensor digitorum longus (EDL), and ventricle was analyzed by pyrophosphate gel electrophoresis. The analogue was found to induce significant alterations in the type of myosin expressed in soleus and EDL. Normal soleus contains both intermediate (IM) and slow (SM) myosins, and treatment reduced the relative content of IM by approximately 50%. In EDL, treatment decreased fast isomyosin FM3 by 60% compared with controls. Sodium dodecyl sulfate-gel electrophoresis also showed a decrease of parvalbumin in EDL by approximately 50%. Treatment had no significant effect on the isomyosin composition of heart ventricle. Levels of physical activity and concentrations of serum glucose and thyroxine of treated mice were not significantly different from controls. These results indicate a role for intracellular energetics in mediating adaptive changes in the phenotype of muscle in mature animals.

This article has been cited by other articles:

				J. S. Otis, R. R. Roy, V. R. Edgerton, and R. J. Talmadge Adaptations in metabolic capacity of rat soleus after paralysis J Appl Physiol, February 1, 2004; 96(2): 584 - 596. [Abstract] [Full Text] [PDF]

				J. Stoppani, A. L. Hildebrandt, K. Sakamoto, D. Cameron-Smith, L. J. Goodyear, and P. D. Neufer AMP-activated protein kinase activates transcription of the UCP3 and HKII genes in rat skeletal muscle Am J Physiol Endocrinol Metab, December 1, 2002; 283(6): E1239 - E1248. [Abstract] [Full Text] [PDF]

				P. E. Durante, K. J. Mustard, S.-H. Park, W. W. Winder, and D. G. Hardie Effects of endurance training on activity and expression of AMP-activated protein kinase isoforms in rat muscles Am J Physiol Endocrinol Metab, July 1, 2002; 283(1): E178 - E186. [Abstract] [Full Text] [PDF]

				M. Wyss and R. Kaddurah-Daouk Creatine and Creatinine Metabolism Physiol Rev, July 1, 2000; 80(3): 1107 - 1213. [Abstract] [Full Text] [PDF]

				M. van Leemputte, K. Vandenberghe, and P. Hespel Shortening of muscle relaxation time after creatine loading J Appl Physiol, March 1, 1999; 86(3): 840 - 844. [Abstract] [Full Text] [PDF]

				P. C. Tullson, J. W. E. Rush, B. Wieringa, and R. L. Terjung Alterations in AMP deaminase activity and kinetics in skeletal muscle of creatine kinase-deficient mice Am J Physiol Cell Physiol, May 1, 1998; 274(5): C1411 - C1416. [Abstract] [Full Text] [PDF]

				J. W. E. Rush, P. C. Tullson, and R. L. Terjung Molecular and kinetic alterations of muscle AMP deaminase during chronic creatine depletion Am J Physiol Cell Physiol, February 1, 1998; 274(2): C465 - C471. [Abstract] [Full Text] [PDF]

				T. Tanaka, Y. Ohira, M. Danda, H. Hatta, and I. Nishi Improved fatigue resistance not associated with maximum oxygen consumption in creatine-depleted rats J Appl Physiol, June 1, 1997; 82(6): 1911 - 1917. [Abstract] [Full Text] [PDF]

				J. F. Watchko, M. J. Daood, G. C. Sieck, J. J. Labella, B. T. Ameredes, A. P. Koretsky, and B. Wieringa Combined myofibrillar and mitochondrial creatine kinase deficiency impairs mouse diaphragm isotonic function J Appl Physiol, May 1, 1997; 82(5): 1416 - 1423. [Abstract] [Full Text] [PDF]