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Am J Physiol Cell Physiol 257: C520-C527, 1989;
0363-6143/89 $5.00
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AJP - Cell Physiology, Vol 257, Issue 3 C520-C527, Copyright © 1989 by American Physiological Society

ARTICLES

Cl-Cl exchange in promyelocytic HL-60 cells follows simultaneous rather than ping-pong kinetics

D. Restrepo, D. J. Kozody, L. J. Spinelli and P. A. Knauf
Department of Biophysics, University of Rochester, School of Medicine and Dentistry, New York 14642.

The intra- and extracellular chloride concentration dependencies of the rate of Cl-Cl exchange in human promyelocytic leukemic HL-60 cells were studied by means of radioactive isotope (36Cl) efflux measurements. Efflux of isotope from cells follows an exponential time course. The Cl-Cl exchange flux follows Michaelis-Menten kinetics as a function of both intra- and extracellular chloride concentrations. The ratio of the maximum exchange velocity to the apparent Michaelis constant for both extracellular and intracellular substrate increases as a function of trans Cl concentration, indicating that Cl-Cl exchange in the HL-60 cell does not follow ping-pong kinetics. A kinetic scheme in which extracellular and intracellular chloride ions bind in random order to the transporter and are then translocated simultaneously can adequately model the experimental data.


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[Abstract] [Full Text] [PDF]


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