Am J Physiol Cell Physiol AJP: Regulatory, Integrative and Comparative Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 257: C495-C503, 1989;
0363-6143/89 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gorin, F.
Right arrow Articles by Carlsen, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gorin, F.
Right arrow Articles by Carlsen, R.

AJP - Cell Physiology, Vol 257, Issue 3 C495-C503, Copyright © 1989 by American Physiological Society

ARTICLES

Abnormal expression of glycogen phosphorylase genes in regenerated muscle

F. Gorin, P. Ignacio, R. Gelinas and R. Carlsen
Department of Neurology, University of California School of Medicine, Davis 95616.

Physiological and molecular biological properties of free, orthotopic grafts of rat extensor digitorum longus (EDL) muscle were determined at 28-, 42-, and 76-days postgraft. cDNA probes for the rat fetal (B), liver (L), and muscle (M) isozymes of glycogen phosphorylase were used to assay isozyme mRNA levels. Regenerating muscle grafts did not express nonmuscle phosphorylase isozymes in vivo in contrast to primary rat skeletal muscle explants in vitro. Low levels of M-phosphorylase mRNA were present at all stages of regeneration in the grafts. However, M-phosphorylase mRNA levels and activity increased markedly and nonuniformly in a subset of functionally and morphologically stabilized regenerated muscle fibers between 42- and 76-days postgraft. Biochemical, physiological, and histochemical characterization of the stabilized grafts demonstrated that all fibers present were innervated and indicated that innervation might be a necessary, but not sufficient, condition for the increase in M-phosphorylase expression. The nonuniform appearance of phosphorylase activity suggests that a differential activity profile imposed on muscle fibers by their motoneuron may govern M-phosphorylase gene expression.


This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
A. Martinuzzi, G. Schievano, A. Nascimbeni, and M. Fanin
McArdle's Disease : The Unsolved Mystery of the Reappearing Enzyme
Am. J. Pathol., June 1, 1999; 154(6): 1893 - 1897.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online