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Am J Physiol Cell Physiol 257: C341-C346, 1989;
0363-6143/89 $5.00
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AJP - Cell Physiology, Vol 257, C341-C346, Copyright © 1989 by the American Physiological Society

Calcium pump epitopes in placental trophoblast basal plasma membranes

James L. Borke 1, Ariel Caride 1, Anil K. Verma 1, Lucky K. Kelley 1, Carl H. Smith 1, John T. Penniston 1, and Rajiv Kumar 1

1 Department of Medicine, Division of Nephrology, and Department of Biochemistry and Molecular Biology, Mayo Clinic and Foundation, Rochester, Minnesota 55905; and Departments of Pediatrics and Pathology, Washington University School of Medicine, St. Louis Childrens Hospital, St. Louis, Missouri 63110

The syncytiotrophoblast represents the primary cellular barrier between maternal and fetal circulations in the placenta. Large amounts of Ca2+ are transported across this barrier by mechanisms that are not clearly understood. To further understand this phenomenon, we examined rat and human placenta by immunohistochemical and protein blotting techniques with a monoclonal antibody raised against the human erythrocyte plasma membrane Ca2+ pump. Immunohistochemistry with this antibody showed specific staining in the human placenta of the basal (fetal facing) surface of the syncytiotrophoblast. In the rat placenta, immunohistochemistry also showed specific staining of the innermost (fetal facing) layer of the trophoblast and the basal surface of the endoderm of the intraplacental yolk sac. In Western blots of placental homogenates and membranes, the monoclonal antibody bound to a 140,000-mol wt band, characteristic of Ca2+ pumps in other tissues. Western blots of isolated basal membranes showed more intense staining than isolated microvillous membranes, confirming the results of the immunohistochemistry. In addition, Ca2+ transport in basal membrane vesicles from human placenta was inhibited by polyclonal antibodies prepared against the erythrocyte Ca2+ pump. We conclude that basal (fetal facing) layers of human and rat placentas contain a high-affinity Ca2+ pump situated to transport Ca2+ from the maternal to the fetal circulation.

calcium-magnesium-adenosinetriphosphatase; calcium transport; immunohistochemistry; rat and human placenta

Submitted on November 14, 1988
Accepted on March 27, 1989




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