AJP - Cell Physiology, Vol 256, Issue 6 C1160-C1167, Copyright © 1989 by American Physiological Society
Local cerebral glucose utilization during status epilepticus in newborn primates
D. G. Fujikawa, B. E. Dwyer, R. R. Lake and C. G. Wasterlain
Epilepsy Research Laboratory, Veterans Administration Medical Center, Sepulveda, California 91343.
The effect of bicuculline-induced status epilepticus (SE) on local cerebral
metabolic rates for glucose (LCMRglc) was studied in 2-wk-old
ketamine-anesthetized marmoset monkeys, using the 2-[14C]-deoxy-D-glucose
autoradiographical technique. To estimate LCMRglc in cerebral cortex and
thalamus during SE, the lumped constant (LC) for 2-deoxy-D-glucose (2-DG)
and the rate constants for 2-DG and glucose were calculated for these
regions. The control LC was 0.43 in frontoparietal cortex, 0.51 in temporal
cortex, and 0.50 in thalamus; it increased to 1.07 in frontoparietal
cortex, 1.13 in temporal cortex, and 1.25 in thalamus after 30 min of
seizures. With control LC values, LCMRglc in frontoparietal cortex,
temporal cortex, and dorsomedial thalamus appeared to increase four to
sixfold. With seizure LC values, LCMRglc increased 1.5- to 2-fold and only
in cortex. During 45-min seizures, LCMRglc in cortex and thalamus probably
increases 4- to 6-fold initially and later falls to the 1.5- to 2-fold
level as tissue glucose concentrations decrease. Together with our previous
results demonstrating depletion of high-energy phosphates and glucose in
these regions, the data suggest that energy demands exceed glucose supply.
The long-term effects of these metabolic changes on the developing brain
remain to be determined.
