Am J Physiol Cell Physiol AJP: Regulatory, Integrative and Comparative Physiology
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Am J Physiol Cell Physiol 256: C994-C1003, 1989;
0363-6143/89 $5.00
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AJP - Cell Physiology, Vol 256, Issue 5 C994-1003, Copyright © 1989 by American Physiological Society

ARTICLES

Role of chloride in potassium transport through a K-Cl cotransport system in human red blood cells

C. Brugnara, T. Van Ha and D. C. Tosteson
Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts.

In this paper, we report experiments demonstrating the coupling of Cl and K movements in a volume-dependent K-Cl cotransport system in human red blood cells. We show that an outwardly directed Cl gradient can promote net K efflux against an inwardly directed K gradient at constant membrane potential. Red blood cell membrane potential was kept constant by using anions that are not transported through the K-Cl cotransport system but that are more permeable than Cl (NO3 and SCN). Under these conditions, when the activities of band 3 (capnophorin)-mediated anion exchange and of the carbonic anhydrase have been inhibited, it is possible to maintain a Cl gradient at constant membrane potential. Similar data were obtained in human red blood cells (least dense fraction from normal subjects and whole blood from patients with homozygous hemoglobin S disease), in rabbit red blood cells, and in low-K sheep red blood cells. These data confirm that the volume-dependent Cl-dependent K movement in these cells operates through coupled K-Cl cotransport.


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