Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 256: C1097-C1104, 1989;
0363-6143/89 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Herbst, M. D.
Right arrow Articles by Goldstein, J. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Herbst, M. D.
Right arrow Articles by Goldstein, J. H.

AJP - Cell Physiology, Vol 256, Issue 5 C1097-C1104, Copyright © 1989 by American Physiological Society

ARTICLES

A review of water diffusion measurement by NMR in human red blood cells

M. D. Herbst and J. H. Goldstein
Department of Chemistry, Emory University, Atlanta, Georgia 30322.

This review of water transport measurement in normal human erythrocytes attempts to harmonize discordant results obtained under diverse study conditions with two different techniques: nuclear magnetic resonance (NMR) and radioactive tracer (THO) diffusion. Natural aggregation of red cells into rouleaux appeared to cause most of the variation among results from NMR experiments. The remainder of the discrepancy was attributed to the use of inappropriate mathematical approximations of the two-site exchange equations, differences in blood storage time, and failure to adjust NMR calculations for the nonwater protons. Differences in hematocrit, frequency-magnetic field strength, or NMR pulse technique played no apparent role in the disparity among NMR reports. When these confounding factors were removed, diffusion results obtained by NMR or by influx or bulk diffusion of radioactive tracer agreed within a relatively narrow range of values. These techniques place the mean lifetime of water inside fresh normal human erythrocytes at room temperature (20-25 degrees C) between the extremes of 9.8 and 14 ms, where the uncorrected range was previously 9.8-21.7 ms. This new range of water exchange times corresponds to a range of diffusional permeability between 3.3 and 4.7 x 10(-3) cm/s.


This article has been cited by other articles:


Home page
 Biophys. JHome page
J. V. Sehy, A. A. Banks, J. J. H. Ackerman, and J. J. Neil
Importance of Intracellular Water Apparent Diffusion to the Measurement of Membrane Permeability
Biophys. J., November 1, 2002; 83(5): 2856 - 2863.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Bifone, Y.-Q. Song, R. Seydoux, R. E. Taylor, B. M. Goodson, T. Pietrass, T. F. Budinger, G. Navon, and A. Pines
NMR of laser-polarized xenon in human blood
PNAS, November 12, 1996; 93(23): 12932 - 12936.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online