Am J Physiol Cell Physiol Journal of Applied Physiology
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Am J Physiol Cell Physiol 256: C1004-C1011, 1989;
0363-6143/89 $5.00
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AJP - Cell Physiology, Vol 256, Issue 5 C1004-C1011, Copyright © 1989 by American Physiological Society

ARTICLES

Isolation and culture of HCO3- -secreting intercalated cells

J. Van Adelsberg, J. C. Edwards, D. Herzlinger, C. Cannon, M. Rater and Q. al-Awqati
Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032.

Intercalated cells of the distal nephron secrete either H+ or HCO3-. We have succeeded in isolating HCO3- -secreting intercalated cells from the rabbit kidney. When seeded onto collagen-coated permeable supports, these cells form monolayers with a resistance of 595 +/- 75 omega.cm2. The monolayers maintain the characteristics of an epithelium, with apical microvillae and tight junctions. They display the same polarity as do HCO3- -secreting intercalated cells in vivo, namely apical peanut lectin binding and apical Cl- -HCO3- exchange. The monolayers are capable of transepithelial HCO3- transport via this exchanger. The rate of Cl- -dependent transepithelial HCO3- transport is 4 +/- 0.4 nmol.min-1.cm-2. Transepithelial HCO3- transport is completely abolished by 50 microM 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid applied to the apical side of the monolayer. These cultured HCO3- -secreting intercalated cells should prove useful for defining the cellular regulation of HCO3- secretion.


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