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Am J Physiol Cell Physiol 255: C526-C530, 1988;
0363-6143/88 $5.00
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AJP - Cell Physiology, Vol 255, Issue 4 C526-C530, Copyright © 1988 by American Physiological Society

ARTICLES

Cytochalasin B inhibition of toad bladder apical membrane responses to ADH

J. B. Wade and W. A. Kachadorian
Department of Physiology, University of Maryland School of Medicine, Baltimore 21201.

The possible role of actin microfilaments in antidiuretic hormone (ADH)-induced increases in apical membrane water permeability was investigated in studies that evaluate inhibition by cytochalasin B of both permeability and membrane structural responses in the toad urinary bladder. Experiments were carried out in the absence of a transepithelial osmotic gradient to eliminate possible flow-induced distortions of the response. Measurements of osmotic water permeability after a brief tissue fixation with glutaraldehyde show that cytochalasin B reduces the permeability response to ADH by approximately one-third. Freeze-fracture electron microscopy indicates that the intramembrane particle aggregates, previously found to correlate closely with ADH-induced permeability, are reduced by about the same extent (28%) under these conditions. However, the frequency of apical membrane fusion events was not affected by cytochalasin B treatment. These results suggest that cytochalasin B treatment in the absence of an osmotic gradient alters the ADH-induced permeability through an effect on apical membrane aggregate frequency.


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