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AJP - Cell Physiology, Vol 255, Issue 2 C214-C225, Copyright © 1988 by American Physiological Society
ARTICLES |
G. E. Revtyak, M. J. Hughes, A. R. Johnson and W. B. Campbell
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235.
Endothelial cells (EC) cultured from human umbilical artery (UA) and vein (UV) metabolized [14C]arachidonic acid to prostaglandins (PGs), monohydroxyeicosatetraenoic acids (HETEs), and epoxyeicosatrienoic acids (EETs). Major radioactive products were identified as 6-keto-PGF1 alpha, PGE2, PGF2 alpha, 12-hydroxy heptadecatrienoic acid, 15-HETE, and 11-HETE. In addition, extracts from UV ECs contained 12-HETE, 5-HETE, 14,15-EET, and 5,6-EET as minor products, whereas extracts from UA ECs contained only 12-HETE as a minor product. UA ECs also produced metabolites comigrating with 14,15-EET, 11,12-EET, 8,9-EET, and 5,6-EET. Histamine increased the release of [14C]PGs and [14C]HETEs from [14C]arachidonic acid-labeled ECs. Indomethacin, aspirin, and nordihydroguauretic acid completely inhibited synthesis of both [14C]PGs and [14C]HETEs from exogenous [14C]arachidonic acid in these cells. Microsomes metabolized [14C]arachidonic acid to the same [14C]PGs and [14C]HETEs as intact cells. Pretreatment of microsomes with indomethacin completely inhibited formation of these products. These data indicate that UA ECs and UV ECs metabolize endogenous and exogenous arachidonic acid to both PGs and HETEs. Also 15-HETE and 11-HETE appear to be synthesized by a microsomal enzyme with the properties of cyclooxygenase.
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 R. J. Roman P-450 Metabolites of Arachidonic Acid in the Control of Cardiovascular Function Physiol Rev, January 1, 2002; 82(1): 131 - 185. [Abstract] [Full Text] [PDF]
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 W. B. Campbell and D. R. Harder Endothelium-Derived Hyperpolarizing Factors and Vascular Cytochrome P450 Metabolites of Arachidonic Acid in the Regulation of Tone Circ. Res., March 5, 1999; 84(4): 484 - 488. [Full Text] [PDF]
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 E. R. Jacobs, R. M. Effros, J. R. Falck, K. M. Reddy, W. B. Campbell, and D. Zhu Airway synthesis of 20-hydroxyeicosatetraenoic acid: metabolism by cyclooxygenase to a bronchodilator Am J Physiol Lung Cell Mol Physiol, February 1, 1999; 276(2): L280 - L288. [Abstract] [Full Text] [PDF]
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P.-L. Li, A.-P. Zou, and W. B. Campbell Regulation of KCa-channel activity by cyclic ADP-ribose and ADP-ribose in coronary arterial smooth muscle Am J Physiol Heart Circ Physiol, September 1, 1998; 275(3): H1002 - H1010. [Abstract] [Full Text] [PDF]
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P. F. Pratt, C. J. Hillard, W. S. Edgemond, and W. B. Campbell N-arachidonylethanolamide relaxation of bovine coronary artery is not mediated by CB1 cannabinoid receptor Am J Physiol Heart Circ Physiol, January 1, 1998; 274(1): H375 - H381. [Abstract] [Full Text] [PDF]
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P.-L. Li and W. B. Campbell Epoxyeicosatrienoic Acids Activate K+ Channels in Coronary Smooth Muscle Through a Guanine Nucleotide Binding Protein Circ. Res., June 19, 1997; 80(6): 877 - 884. [Abstract] [Full Text]
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W. B. Campbell, D. Gebremedhin, P. F. Pratt, and D. R. Harder Identification of Epoxyeicosatrienoic Acids as Endothelium-Derived Hyperpolarizing Factors Circ. Res., March 1, 1996; 78(3): 415 - 423. [Abstract] [Full Text]
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