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Am J Physiol Cell Physiol 255: C12-C18, 1988;
0363-6143/88 $5.00
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AJP - Cell Physiology, Vol 255, Issue 1 C12-C18, Copyright © 1988 by American Physiological Society

ARTICLES

Gene transfer of a putative mammalian K+ channel gene from genomic and cosmid DNA

M. Mitas, C. Coogan and J. J. Gargus
Department of Physiology, Emory University School of Medicine, Atlanta, Georgia 30322.

We have successfully transfected mouse genomic DNA containing a mutant putative K+ channel gene into murine LMTK- cells. Incorporation of the mutant gene allowed primary transformants to express the mutant cell phenotype of growth at a subthreshold K+ concentration and, furthermore, caused these primary transformants to display the same characteristics of growth and unidirectional K+ flux as the mutant cell line, LTK-1, from which the genomic DNA was derived. A cosmid genomic library was formed from LTK-1 mutant DNA and shown by transfection experiments to contain at least one unique cosmid clone containing a functional copy of the mutant putative K+ channel gene.




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