AJP - Cell Physiology, Vol 254, Issue 5 C605-C613, Copyright © 1988 by American Physiological Society

ARTICLES

Functional significance of myosin transitions in single fibers of developing soleus muscle

P. J. Reiser, C. E. Kasper, M. L. Greaser and R. L. Moss
Department of Physiology, School of Medicine, University of Wisconsin, Madison 53706.

The maximal velocity of shortening and myosin heavy chain (MHC) composition of single, chemically skinned fibers from neonatal and adult rat soleus muscles were examined to determine the relationship between these parameters during slow muscle development in the rat. In addition, the MHC composition of bundles of fibers from soleus muscles at the same ages was studied. The MHC compositions were examined using sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. The results from the bundles of fibers indicate that from 3 days to 5 mo postnatal, the rat soleus contains predominantly MHCs that migrate in the vicinity of the MHC from adult slow muscle. From 14 days to 2 mo postnatal, there are also significant amounts of additional MHCs that comigrate on SDS gels with those characteristic of adult rat fast muscle. All the fibers studied at 3 and 7 days postnatal and at 5 mo and the majority of fibers from 14 days to 2 mo postnatal had relatively low shortening velocities. A few fibers from the latter group had significantly higher velocities. The faster fibers at each age had greater amounts of the MHCs that comigrate with the adult fast-type MHC on SDS gels. Thus the velocity of shortening of single fibers from the rat soleus muscle appears to be related to MHC composition during postnatal development.

This article has been cited by other articles:

				J. J. Widrick, G. F. Maddalozzo, H. Hu, J. C. Herron, U. T. Iwaniec, and R. T. Turner Detrimental effects of reloading recovery on force, shortening velocity, and power of soleus muscles from hindlimb-unloaded rats Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2008; 295(5): R1585 - R1592. [Abstract] [Full Text] [PDF]

				P. C. Geiger, J. P. Bailey, C. B. Mantilla, W.-Z. Zhan, and G. C. Sieck Mechanisms underlying myosin heavy chain expression during development of the rat diaphragm muscle J Appl Physiol, December 1, 2006; 101(6): 1546 - 1555. [Abstract] [Full Text] [PDF]

				C. Coirault, B. Pignol, R. N. Cooper, G. Butler-Browne, P.-E. Chabrier, and Y. Lecarpentier Severe muscle dysfunction precedes collagen tissue proliferation in mdx mouse diaphragm J Appl Physiol, May 1, 2003; 94(5): 1744 - 1750. [Abstract] [Full Text] [PDF]

				G. C. Sieck, Y. S. Prakash, Y.-S. Han, Y.-H. Fang, P. C. Geiger, and W.-Z. Zhan Changes in actomyosin ATP consumption rate in rat diaphragm muscle fibers during postnatal development J Appl Physiol, May 1, 2003; 94(5): 1896 - 1902. [Abstract] [Full Text] [PDF]

				L. Yang, J. Luo, J. Bourdon, M.-C. Lin, S. B. Gottfried, and B. J. Petrof Controlled Mechanical Ventilation Leads to Remodeling of the Rat Diaphragm Am. J. Respir. Crit. Care Med., October 15, 2002; 166(8): 1135 - 1140. [Abstract] [Full Text] [PDF]

				F. Sasarman, G. Karpati, and E. A. Shoubridge Nuclear genetic control of mitochondrial translation in skeletal muscle revealed in patients with mitochondrial myopathy Hum. Mol. Genet., July 1, 2002; 11(14): 1669 - 1681. [Abstract] [Full Text] [PDF]

				R. G. Dennis, P. E. Kosnik II, M. E. Gilbert, and J. A. Faulkner Excitability and contractility of skeletal muscle engineered from primary cultures and cell lines Am J Physiol Cell Physiol, February 1, 2001; 280(2): C288 - C295. [Abstract] [Full Text] [PDF]

				G. C. Sieck and W.-Z. Zhan Denervation alters myosin heavy chain expression and contractility of developing rat diaphragm muscle J Appl Physiol, September 1, 2000; 89(3): 1106 - 1113. [Abstract] [Full Text] [PDF]

				W.-Z. Zhan, J. F. Watchko, Y. S. Prakash, and G. C. Sieck Isotonic contractile and fatigue properties of developing rat diaphragm muscle J Appl Physiol, April 1, 1998; 84(4): 1260 - 1268. [Abstract] [Full Text] [PDF]

				J. J. Widrick, J. G. Romatowski, J. L. W. Bain, S. W. Trappe, T. A. Trappe, J. L. Thompson, D. L. Costill, D. A. Riley, and R. H. Fitts Effect of 17 days of bed rest on peak isometric force and unloaded shortening velocity of human soleus fibers Am J Physiol Cell Physiol, November 1, 1997; 273(5): C1690 - C1699. [Abstract] [Full Text] [PDF]

				C. COIRAULT, F. LAMBERT, T. JOSEPH, F.-X. BLANC, D. CHEMLA, and Y. LECARPENTIER Developmental Changes in Crossbridge Properties and Myosin Isoforms in Hamster Diaphragm Am. J. Respir. Crit. Care Med., September 1, 1997; 156(3): 959 - 967. [Abstract] [Full Text] [PDF]