Am J Physiol Cell Physiol AJP: Heart and Circulatory Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 253: C817-C827, 1987;
0363-6143/87 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Takuwa, Y.
Right arrow Articles by Rasmussen, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Takuwa, Y.
Right arrow Articles by Rasmussen, H.

AJP - Cell Physiology, Vol 253, Issue 6 C817-C827, Copyright © 1987 by American Physiological Society

ARTICLES

Measurement of cytoplasmic free Ca2+ concentration in bovine tracheal smooth muscle using aequorin

Y. Takuwa, N. Takuwa and H. Rasmussen
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510.

The cytoplasmic free Ca2+ concentration in bovine tracheal smooth muscle strips was measured using aequorin. Carbachol induces a rapid rise in aequorin luminescence, which reaches a peak within 1-2 min and then falls to a plateau level in 5-6 min. This plateau gradually declines but remains significantly above the base-line value after 2 h. The initial Ca2+ transient is due to Ca2+ mobilization from an intracellular caffeine-sensitive pool. The plateau appears to be due to Ca2+ influx. Histamine or 5-hydroxytryptamine (serotonin) also induce a Ca2+ transient followed by a plateau phase which is lower than that induced by carbachol. The isometric tension generated by either of these two agonists shows a gradual decline in contrast to the sustained plateau seen in the carbachol-induced contraction. Extracellular high K+ induces a dose-dependent increase in aequorin luminescence that is totally due to Ca2+ influx across the plasma membrane and is greatly inhibited by a Ca2+-channel antagonist, nimodipine. These results suggest that two temporally and spatially different Ca2+-dependent mechanisms are involved in carbachol-induced tracheal smooth muscle contraction.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
P. H. Ratz, K. M. Berg, N. H. Urban, and A. S. Miner
Regulation of smooth muscle calcium sensitivity: KCl as a calcium-sensitizing stimulus
Am J Physiol Cell Physiol, April 1, 2005; 288(4): C769 - C783.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
S. J. Hill, C. R. Ganellin, H. Timmerman, J. C. Schwartz, N. P. Shankley, J. M. Young, W. Schunack, R. Levi, and H. L. Haas
International Union of Pharmacology. XIII. Classification of Histamine Receptors
Pharmacol. Rev., September 1, 1997; 49(3): 253 - 278.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
H. Karaki, H. Ozaki, M. Hori, M. Mitsui-Saito, K.-I. Amano, K.-I. Harada, S. Miyamoto, H. Nakazawa, K.-J. Won, and K. Sato
Calcium Movements, Distribution, and Functions in Smooth Muscle
Pharmacol. Rev., June 1, 1997; 49(2): 157 - 230.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online