AJP - Cell Physiology, Vol 251, Issue 6 935-C944, Copyright © 1986 by American Physiological Society
Isolated canine mastocytoma cells: propagation and characterization of two cell lines
S. C. Lazarus, R. DeVinney, L. J. McCabe, W. E. Finkbeiner, D. J. Elias and W. M. Gold
Five different dog mastocytoma tumors were successfully transplanted and
propagated in BALB/c nude mice. Cells from two of these tumors were
passaged serially through at least four generations of mice without
morphological or functional change. The average yield from a 2-cm tumor
harvested from a mouse was 1.2 +/- 2.8 X 10(9) mast cells with greater than
90% viability. Cells of one line were larger and more heavily granulated
than the other, and contained 1.29 +/- 0.74 pg histamine/cell (mean +/-
SD). Calcium ionophore A23187 and compound 48/80 caused dose dependent
histamine release with no significant difference in release from generation
to generation. The smaller cells contained 0.06 +/- 0.06 pg histamine/cell.
Histamine release after calcium ionophore or compound 48/80 was dose
dependent and unchanged through serial passages. Following passive
sensitization antigen caused dose-dependent histamine release confirming
the presence of IgE receptors on these cells. In both cell lines histamine
release was inhibited by terbutaline, dibutyryl adenosine 3',5'-cyclic
monophosphate, or isobutylmethylxanthine. These methods provide a
morphologically and functionally stable population of nearly pure canine
mast cells for biochemical and physiological studies.
