Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 248: C177-C180, 1985;
0363-6143/85 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lin, L. E.
Right arrow Articles by Civan, M. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lin, L. E.
Right arrow Articles by Civan, M. M.

AJP - Cell Physiology, Vol 248, Issue 1 177-C180, Copyright © 1985 by American Physiological Society

ARTICLES

31P-nuclear magnetic resonance analysis of perfused single frog skins

L. E. Lin, M. Shporer and M. M. Civan

Perfusion of single frog skins has produced stability of the 31P-nuclear magnetic resonance (NMR) spectra over periods as long as 8 h at room temperature. With this approach, relatively large phosphocreatine (PCr) signals were recorded for each of the 12 frog skins studied. The ratio of the concentration of PCr to ATP was estimated to be 0.76 +/- 0.07, a value an order of magnitude larger than that previously reported. Comparison of the ratio of the intracellular concentrations of inorganic phosphate (Pi) to ATP determined in the present NMR study with that previously estimated by chemical analysis suggests that little intracellular Pi is likely to be immobilized. Inclusion of methylphosphonate in the perfusing solutions permitted simultaneous determination of extracellular and intracellular pH from the NMR spectra alone. The methylphosphonate has been found to be nontoxic. At an extracellular pH of 7.72 +/- 0.04, the intracellular pH was 7.47 +/- 0.06.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. Pietri, S. Martel, M. Culcasi, M.-C. Delmas-Beauvieux, P. Canioni, and J.-L. Gallis
Use of Diethyl(2-methylpyrrolidin-2-yl)phosphonate as a Highly Sensitive Extra- and Intracellular 31P NMR pH Indicator in Isolated Organs. DIRECT NMR EVIDENCE OF ACIDIC COMPARTMENTS IN THE ISCHEMIC AND REPERFUSED RAT LIVER
J. Biol. Chem., January 12, 2001; 276(3): 1750 - 1758.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online