Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 244: C422-C428, 1983;
0363-6143/83 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Piwnica-Worms, D.
Right arrow Articles by Lieberman, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Piwnica-Worms, D.
Right arrow Articles by Lieberman, M.

AJP - Cell Physiology, Vol 244, Issue 5 422-C428, Copyright © 1983 by American Physiological Society

ARTICLES

Microfluorometric monitoring of pHi in cultured heart cells: Na+-H+ exchange

D. Piwnica-Worms and M. Lieberman

Continuous measurement of intracellular pH (pHi) should enhance the likelihood of defining the mechanisms of pHi regulation in actively contracting preparations of cardiac muscle. A filter microfluorometric technique was adapted for use with growth-oriented embryonic chick heart cells to continuously monitor changes in the fluorescence intensity of the pH-sensitive chromophore 6-carboxyfluorescein, generated in situ. Data pertaining to the direction and the rate of pHi changes assisted in thermodynamically characterizing and ascertaining net kinetic parameters of a Na+-H+ exchange mechanism. Imposing an outward Na+ gradient across the cardiac cell membrane rapidly (t 1/2 = 44 s) induced cytosolic acidification, whereas an inward Na+ gradient produced cytosolic alkalinization (t 1/2 = 40 s). Amiloride (10(-3) M) caused the cytoplasm to acidify (t 1/2 = 46 s) and also reversibly blocked the acidification induced by low extracellular Na+. These results are consistent with the presence of a rapid Na+-H+ exchange mechanism in the cardiac cell membrane. Further investigations are required to characterize the involvement of Na+-H+ exchange in pHi regulation and to differentiate the effects of Na+-H+ exchange from other ion gradient-coupled mechanisms, e.g., Na+-Ca2+ exchange.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. P. Goel, A. Vecchini, V. Panagia, and G. N. Pierce
Altered cardiac Na+/H+ exchange in phospholipase D-treated sarcolemmal vesicles
Am J Physiol Heart Circ Physiol, September 1, 2000; 279(3): H1179 - H1184.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
I. Korichneva, M. Puceat, R. Cassoly, and G. Vassort
Cl--HCO3- Exchange in Developing Neonatal Rat Cardiac Cells : Biochemical Identification and Immunolocalization of Band 3–Like Proteins
Circ. Res., September 1, 1995; 77(3): 556 - 564.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online