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Am J Physiol Cell Physiol 242: C382-C387, 1982;
0363-6143/82 $5.00
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AJP - Cell Physiology, Vol 242, Issue 5 382-C387, Copyright © 1982 by American Physiological Society

ARTICLES

Glucose-induced proton uptake in secretory granules of beta-cells in monolayer culture

C. S. Pace and G. Sachs

The weak base acridine orange (AO) has been shown to be accumulated by the insulin-containing secretory granules of cultured beta-cells in response to high glucose. Various lines of evidence indicate that this accumulation is due to a pH gradient. Thus ionophores such as monensin and nigericin abolish the glucose-induced accumulation, and a high concentration of the weak base, benzylamine, results in swelling of the granules. In the absence of glucose, ATP addition to digitonin-permeabilized cells also results in dye uptake. These data also suggest that a primary or secondary active accumulation mechanism for hydrogen ions exists across the granule membrane.


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L. S. Tompkins, K. D. Nullmeyer, S. M. Murphy, C. S. Weber, and R. M. Lynch
Regulation of secretory granule pH in insulin-secreting cells
Am J Physiol Cell Physiol, August 1, 2002; 283(2): C429 - C437.
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