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Fig. 2.   ROS alter expression of extracellular matrix mRNAs. A: representative Northern hybridization showing decreased expression of procollagen alpha 1(I), alpha 2(I), and alpha 1(III) mRNA and differential expression of procollagen alpha 1(IV) and alpha 2(IV) and fibronectin mRNA with 24-h treatment of neonatal cardiac fibroblasts with H2O2 (5 µM) or XXO (500 µM + 0.1 mU/ml). The level of 18S mRNA was used to control for differences in loading and transfer. B: quantification of the procollagen alpha 1(I), alpha 2(I), alpha 1(III), alpha 1(IV), alpha 2(IV) and fibronectin mRNA level changes in fibroblasts exposed to H2O2 or XXO. Data are presented as the percent change from control; means ± SE of 8-15 experiments. *P <=  0.05 vs. control.